Coding

Part:BBa_J100132:Design

Designed by: Malcolm Campbell   Group: Campbell M Lab   (2013-09-23)


E. coli thyA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 840
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 841
    Illegal PstI site found at 855
    Illegal NotI site found at 7
    Illegal NotI site found at 848
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 841
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 841
    Illegal PstI site found at 855
    Illegal AgeI site found at 637
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 23
    Illegal BsaI.rc site found at 834


Design Notes

We added BioBrick prefix and suffix since the gene was synthesized by GeneArt and cloned into their pMK-T plasmid. There are no internal EcoRI, XbaI, SpeI, PstI, or BsaI restriction sites.


Source

Based on the cells used in this paper by Thompson et al., 2002. [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC139998/ Thompson et al., 2002]

References